Thermo Scientific™ DyLight™ 488 NHS Ester

Description

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.

Thermo Scientific™ Amine-Reactive DyLight Dyes are NHS ester-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods.

The DyLight Dyes exhibit higher fluorescence intensity and photostability than Alexa™, CyDye™ and LI-COR™ Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight Dyes allows a high dye-to-protein ratio without precipitation during conjugation.

Highlights:

• Brightness: high-fluorescence intensity provides outstanding sensitivity and requires less conjugate

• Photostability: exceptional resistance to photobleaching enables fluorescence imaging under the most demanding conditions (e.g., structured illumination, 4pi microscopy)

• Instrument compatibility: emission spectra match the principal output wavelengths of common fluorescence instrumentation

Specifications

• Quantity: 1 mg
• Product Type: Dye
• Solubility: DMF (Dimethylformamide)
• Content And Storage: Store desiccated at -20°C, protected from light.
• Chemical Reactivity: Amine
• Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
• Label or Dye: DyLight™ 488
• Label Type: DyLight Fluors
• Product Line: DyLight™
• Emission: 518
• Excitation: 493
• Labeling Target: Proteins

For Research Use Only. Not for use in diagnostic procedures.