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Thermo Scientific™ DyLight™ 650 Maleimide

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

Supplier:  Thermo Scientific™ 62295

Catalog No. PI62295

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Thermo Scientific™ Sulfhydryl-Reactive DyLight Dyes are maleimide-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as probes for cellular imaging and other fluorescence detection methods.

The Thermo Scientific™ DyLight™ Dyes have absorption spectra ranging from 350 to 770nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor™ or Cy™ Dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight Reagents allows a high fluor-to-protein ratio without precipitation during conjugation.
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ∼1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.

  • Specific — maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods — well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure —complete protocol for IgG reduction and labeling and calculating the labeling efficiency

  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies


DyLight 650 Maleimide
Store desiccated at -20°C. Shipped at ambient temperature.
DyLight Fluors
1 mg
DMF (Dimethylformamide)
DyLight™ 650


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