Invitrogen™ Dynabeads™ FlowComp™ Human CD3 Kit

Description

You can expect a purity level of >96-99% and above 60% recovery of human CD3+ cells.

Dynabeads™ FlowComp™ Human CD3 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD3⁺ T-cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp™ Release Buffer.

• Rapid isolation of CD3⁺ T-cells from any sample – no columns required
• Pure and bead-free CD3⁺ T-cells for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells

Dynabeads™ FlowComp™ Human CD3 contains uniform, superparamagnetic beads (2.8 μm diameter) that enable easy isolation of human CD3⁺ cells directly from any sample via a three-step process. First, the FlowComp™ Human CD3 Antibody is mixed with the sample in a tube where it binds to the target cells during a short incubation. Then, the CD3⁺ T-cells that have bound the specific antibodies are captured by the FlowComp™ Dynabeads™ and separated from unbound cells using a magnet. Finally, the target cells are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD3⁺ cells. The cells can be used in any downstream application.

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Specifications

• Cell Type: T Cells (CD3)
• Content And Storage: The Kit Contains:
  • 1 mL Antibody Mix
  • 3 mL FlowComp™ Dynabeads™
  • 40 mL FlowComp™ Release Buffer.
  
  Store at: 2°C to 8°C.
• Product Type: Superparamagnetic Beads
• Final Product Type: Cells
• Format: Bead
• Isolation Technology: Positive Isolation
• No. of Cells: 80 mL whole blood, or 2 x 10⁹ PBMC
• Product Line: DYNAL™, Dynabeads™, FlowComp™
• Purity or Quality Grade: Research Grade, 99%
• Quantity: 1 Kit
• Sample Type: MNC, Blood
• Shipping Condition: Room Temperature
• Target Species: Human
• Diameter (Metric): 2.8 μm

For Research Use Only. Not for use in diagnostic procedures.