Invitrogen™ Dynabeads™ mRNA DIRECT™ Purification Kit

Catalog No.	Quantity
61-012	10 mL
61-011-LS	5 mL

Catalog No. 61-012 Supplier Invitrogen™ Supplier No. 61012

Description

Includes

10mL Dynabeads oligo (dT)₂₅, 60mL Lysis/Binding Buffer, 120mL Washing Buffer A, 60mL Washing Buffer B, 15mL 10mM Tris-HCl (Elution buffer), Contains enough reagents for 40 standard isolations.

The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues.

The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications.

Advantages of the Dynabeads mRNA DIRECT Kit:
• Fast—15 minute procedure yields pure, intact mRNA
• Highly pure mRNA isolation—best choice upstream of cDNA synthesis
• Sensitive mRNA isolation—enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)

System overview
The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)₂₅ residues covalently coupled to the surface of the Dynabeads. Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol). 1 mg of Dynabeads Oligo (dT)₂₅ beads (200 μL) binds up to 2 μg of mRNA. A typical mammalian cell contains about 10–30 pg of total RNA, from which 1–5% is mRNA.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  • 10 mL Dynabeads Oligo (dt)₂₅, 4°C
  • 60 mL Lysis/Binding Buffer; 4°C
  • 120 mL Washing Buffer A; 4°C
  • 60 mL Washing Buffer B; 4°C
  • 15 mL 10 mM Tris-HCl; 4°C
  
  

• Isolation Technology: Magnetic Bead
• Elution Volume: 10 to 100 μL
• Sample Type: Cells, Plants, Plasma, Serum, Tissue, Total RNA, Blood
• Final Product Type: mRNA
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Equipment): Automated Liquid Handling Systems
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, microarray analysis
• Green Features: Beads may be reused for multiple extractions
• Quantity: 10 mL
• Shipping Condition: Room Temperature
• Starting Material Amount: Cells: ≤10⁶
  Plant: ≤400 mg
  Tissue: ≤200 mg
• Yield: 2 μg mRNA per 200 μL of beads (Binding capacity)

For Research Use Only. Not for use in diagnostic procedures.