Invitrogen™ Dynabeads™ RNA Binding Buffer

Description

Dynabeads RNA Purification Buffer is intended for use with Dynabeads Carboxylic Acid for RNA Purification, providing a simple, scalable, and automation-ready solution for purification of crude RNA produced by in vitro transcription (IVT).

Dynabeads RNA Binding Buffer is intended for use with Dynabeads Carboxylic Acid for RNA Purification beads, together providing a simple, scalable, and automatable solution for purification of crude RNA produced by in vitro transcription (IVT). The products may be used for purification following solid-phase IVT with Dynabeads Streptavidin for In Vitro Transcription (figure below). The combination is particularly suitable where flexibility and high purity are required, such as in the discovery phase of RNA-based vaccines and therapeutics development.

Features of Dynabeads RNA Binding Buffer
This buffer, combined with Dynabeads Carboxylic Acid for RNA Purification, offers efficiency, scalability (micrograms to grams) in manual and automated workflows, and quality to navigate from RNA-based drug discovery to commercial manufacturing without incurring extra time or costs from switching technology or raw material.

The buffer is offered as a stand-alone product. It is also included in the Dynabeads RNA Purification and Dynabeads In Vitro Transcription and RNA Purification kits.

Overview of the workflow
Crude RNA from an IVT reaction is mixed with the beads (not included) and the buffer is added. The RNA binds to the bead surface and the remaining components of the reaction mixture are removed by applying a magnet (see thermofisher.com/magnets) and discarding the supernatant (figure below).

Automation-ready for use with KingFisher purification systems
The 1-μm magnetic beads are ideal for high-throughput screening. The process can be automated using liquid handlers or any of our KingFisher sample purification systems. The scripts for KingFisher Flex and KingFisher Apex automated purification workflows are available from our website.

Commercial supply
To learn more about counterpart products and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics, visit: thermofisher.com/dynabeadsmrna.

Specifications

• Bacterial Content: <10 CFU/mL
• Content And Storage: Store at 2°C to 8°C and protected from light.
• Isolation Technology: Magnetic Bead
• Purification Time: Variable
• Sample Type: Crude RNA
• High-throughput Compatibility: Yes
• For Use With (Equipment): KingFisher™ Sample Purification System, DynaMag™ magnets
• For Use With (Application): In Vitro Transcription, Synthetic RNA, Crude RNA Purification (from IVT Reaction Mix)
• Product Line: Dynabeads™
• Quantity: 50 mL
• RNase: No detectable activity level
• Shipping Condition: Ambient Temperature
• Target: RNA
• Volume (Metric): 50 mL