Invitrogen™ Dynabeads™ Sequential Protein Binding Beads, 100 rxns

Catalog No.	Quantity
50-001-D	2 mL

Catalog No. 50-001-D Supplier Invitrogen™ Supplier No. 50001D

Description

Dynabeads Sequential Protein Binding Beads are hydrophobic 2.8-mm monosized Dynabeads magnetic beads optimized for high binding capacity of proteins from cell lysate for western or mass spectrometry analysis. The beads can be used with Sequential Lysis Buffer, Sequential Protein Bind/Wash Solution, and the Sequential Protein Elution Buffer. It is a component of the Sequential Protein/DNA/RNA Extraction Kit.

Invitrogen Dynabeads Sequential Protein Binding Beads are hydrophobic 2.8-mm monosized Dynabeads magnetic beads optimized for high binding capacity of proteins from cell lysate for western or mass spectrometry analysis. The beads can be used with Sequential Lysis Buffer, Sequential Protein Bind/Wash Solution, and the Sequential Protein Elution Buffer. It is a component of the Sequential Protein/DNA/RNA Extraction Kit.

Features of the Sequential Protein/DNA/RNA Extraction Kit include:

• High sensitivity—supports scalable extractions from precious or low sample volumes down to 1,000 cells or 0.005 mg tissue (tissue dependent)
• Direct compatibility—extracted analytes down to 100,000 cells or 1-2 mg tissue are validated and suitable for mass spectrometry and NGS applications
• Automated high-throughput—compatible with KingFisher instruments for scalable processing
• Enhanced reproducibility—standardized protocol reduces variability

The kit employs a bead-based sequential extraction methodology utilizing protein isolation prior to nucleic acid extraction, resulting in enhanced protein yields that are directly compatible with mass spectrometry analysis. This approach enables researchers to obtain analytes suitable for diverse downstream applications including SDS-PAGE, western blotting, mass spectrometry, sequencing, genotyping, and qPCR from sample inputs as small as 1-2 mg of tissue or 100,000 cells while maintaining quality standards required for both next-generation sequencing (NGS) and MS platforms. The Dynabeads and MagMAX magnetic bead-based purification technology helps enable high reproducibility and scalable, high-throughput processing when integrated with automation systems such as the KingFisher Sample Purification System.

This kit combines the advantages of multi-analyte extraction with the precision and efficiency required for advanced proteomics and genomics workflows, making it particularly valuable for multi-omics studies where sample conservation and analytical comprehensiveness are paramount.

Verified performance

The kit helps deliver yield and quality metrics comparable to standard single-analyte extraction kits, facilitating efficient multi-analyte recovery from a single sample. While most commercial sequential protocols are optimized for large sample volumes (e.g. 1 million cells or 10–20 mg tissue), this kit offers exceptional versatility across a wider range. Validated for 100,000 cells and 1–2 mg tissue, it has also been successfully used with as few as 1,000 cells while maintaining sufficient DNA integrity number (DIN) and RNA integrity number (RIN) yields. This broad dynamic range provides greater flexibility and enables efficient processing of precious or limited samples that are difficult to analyze with conventional methods.

Streamlined three-step sequential extraction

The extraction procedure follows a systematic three-step process designed to support a high recovery of each biomolecule class. Initially, Dynabeads Protein Beads are introduced to the lysed sample and incubated to facilitate protein binding, after which they are transferred to a separate tube for washing and protein processing compatible with downstream western blot or mass-spectrometry applications. Subsequently, MagMAX DNA/RNA Binding beads are added to the original tube, now depleted of proteins, to enable DNA binding and extraction using proprietary buffer formulations before transferring to a new tube for further preparation. Finally, MagMAX DNA/RNA Binding beads are again introduced to the original tube, now depleted of both proteins and DNA, to facilitate RNA binding and extraction using specialized proprietary buffers and solutions, with subsequent transfer to a dedicated tube for final preparation. The resulting extracted proteins are suitable for WB or MS analysis, while the recovered DNA and RNA are compatible with quantitative PCR (qPCR) and next-generation sequencing (NGS) applications.

Specifications

• Formulation: Resin Slurry
• Material: Magnetic Polystyrene
• Content And Storage: •The vial contains 2.8 mm sized Dynabeads with a hydrophobic outer surface optimized for protein binding. Supplied as 100 mg/mL inn water.
  •Store at 2°C to 8°C.
• For Use With (Equipment): KingFisher Duo Prime, Flex, or Apex Purification Systems
• Shipping Condition: Ambient temperature
• Sample Type: Cell lines, PBMC, fresh/frozen tissue digests
• Particle Size: 1 to 3 μm
• Product Line: DYNAL, Dynabeads, MPC
• Quantity: 2 mL
• For Use With (Application): Mass spectrometry, western blot, qPCR, sequencing
• Type: Magnetic Bead Protein Purification System
• Sufficient For: 100 Reactions
• Format: Magnetic Bead
• Regulatory Status: For Research Use Only

For Research Use Only. Not for use in diagnostic procedures.