Invitrogen™ Dynabeads™ Sheep anti-Rat IgG

Description

The product can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed.

Dynabeads Sheep Anti-Rat IgG are 4.5 μm superparamagnetic beads with affinity purified polyclonal sheep anti-rat IgG covalently bound to the bead surface. These beads can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed. Dynabeads Sheep Anti-Rat IgG are especially well suited for cell isolation from mouse samples (cross-reactivity to mouse antibodies is high and cross-reactivity to human antibodies is minimal).

Advantages of Dynabeads Sheep Anti-Rat IgG:

• Works with any cell type, from any sample and from any species (except rat)
• Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody
• Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells

Magnetic bead-based separation offers easy handling

The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads Sheep Anti-Rat IgG (direct technique). Regardless of which technique is used, when the cell sample and the Dynabeads Sheep Anti-Rat IgG are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation). For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads FlowComp Flexi Kit.

Useful for many sample types

Using Dynabeads Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests.

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Specifications

• Antibody Fragment: Whole antibody
• Cell Type: All cells from all species, except rat
• Content And Storage: This Product Contains: 2 mL of Dynabeads™ Sheep Anti-Rat IgG (4 x 108 Dynabeads™/mL) in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide.
• Color: Brown
• For Use With (Application): Cell Depletion, Negative Isolation, Positive Isolation
• Material: Magnetic Polystyrene
• Product Type: Antibody Coated Bead
• No. of Cells: Processes ∼8x 10^8 cells total
• Output Viability: >95%
• Product Line: Dynabeads™
• Purity or Quality Grade: Research Grade
• Quantity: 2 mL
• Sample Type: PBMC, Tissue Digests, Blood
• Shipping Condition: Room Temperature
• Starting Material Cell No.: 1 x 10^7 PBMCs per isolation
• Target Species: All species, except rat
• Diameter (Metric): 4.5 μm

For Research Use Only. Not for use in diagnostic procedures.