TOPOGEN INC E. coli DNA Gyrase

Supplier:  TOPOGEN INC TG2000G3

Contains purified bacterial (E. coli) DNA Gyrase purified to homogeneity (on SDS-PAGE). DNA Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex. The enzyme is supplied at the unit concentration given on the above sticker (in storage buffer: 50 mM Tris-Cl pH 7.5, 100 mM KCl, 2 mM dithiotreitol, 1 mM EDTA, 50% glycerol). A test for nuclease contamination was carried out by assaying for the formation of linear KDNA and linear plasmid DNA. Incubations of 1 μg of catenated KDNA or supercoiled pUC19 DNA (4 hrs. at 37° in the presence of 10 mM MgCl2) were performed. Linear DNA or breakdown products were not generated under these conditions. The A and B subunits are >95% pure based upon SDS-PAGE and certified to be endonuclease free.

Catalog No. NC2216104