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Apexbio Technology LLC E.-COLI-DNA-LIGASE-200U
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Catalog No. 5000567697
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E coli DNA ligase is an enzyme used extensively in molecular biology research particularly within the fields of genetic engineering and biotechnology Originating from the bacterium Escherichia coli this enzyme plays a crucial role in DNA replication and repair by catalyzing the formation of phosphodiester bonds between the 3 -hydroxyl and 5 -phosphate ends of DNA fragments Its mechanism of action involves the joining of Okazaki fragments on the lagging strand during DNA replication as well as sealing nicks in the DNA backbone It exhibits high activity in in vitro applications typically within the nanomolar to low micromolar range E coli DNA ligase is commonly used in applications involving cell models animal studies and drug screening protocols where its ability to ligate DNA fragments is essential Experimental concentrations typically depend on the specific research design allowing for flexibility in various experimental setups

Catalog No. 50-005-67697 Supplier Apexbio Technology LLC Supplier No. K3154200
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E coli DNA ligase is an enzyme used extensively in molecular biology research particularly within the fields of genetic engineering and biotechnology Originating from the bacterium Escherichia coli this enzyme plays a crucial role in DNA replication and repair by catalyzing the formation of phosphodiester bonds between the 3 -hydroxyl and 5 -phosphate ends of DNA fragments Its mechanism of action involves the joining of Okazaki fragments on the lagging strand during DNA replication as well as sealing nicks in the DNA backbone It exhibits high activity in in vitro applications typically within the nanomolar to low micromolar range E coli DNA ligase is commonly used in applications involving cell models animal studies and drug screening protocols where its ability to ligate DNA fragments is essential Experimental concentrations typically depend on the specific research design allowing for flexibility in various experimental setups

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