Invitrogen™ Human E-selectin (Soluble) ELISA Kit

Catalog No.	Quantity
BMS2052TEN	10 x 96 Tests
BMS2052	96 Tests

Catalog No. BMS2052TEN Supplier Invitrogen™ Supplier No. BMS2052TEN

Description

ELISA

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Specifications

• Accession Number: P16581
• Assay Range: 1.6-50 ng/mL
• Assay Sensitivity: 0.017 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CD62 antigen-like family member E; CD62E; e selectin; Elam; ELAM1; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule; endothelial leukocyte adhesion molecule 1; endothelial leukocyte adhesion molecule I; ESEL; E-selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; SELE; selectin E; selectin E (endothelial adhesion molecule 1); selectin, endothelial cellCD62 antigen-like family member E; CD62E; e selectin; Elam; ELAM1; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule; endothelial leukocyte adhesion molecule 1; endothelial leukocyte adhesion molecule I; ESEL; E-selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; SELE; selectin E; selectin E (endothelial adhesion molecule 1); selectin, endothelial cell; CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE
• Gene ID (Entrez): 6401
• Gene Symbol: SELE
• Interassay CV: 3.3%
• Intraassay CV: 5.7%
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Sample diluent, Biotinylated Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate,SAV-HRP,TMB, Stop solution, Adhesive Plate Covers, Standard (2 vials), Sample diluent, Biotinylated Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, SAV-HRP, TMB, Stop solution, Adhesive Plate Covers
• Label or Dye: HRP
• Protein Aliases: CD62 antigen-like family member E, CD62E, E-selectin, ELAM-1, endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, LECAM2, leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 20 μL, Supernatant 20 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: E-selectin (CD62E)
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 30 min