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  5. Invitrogen™ eBioscience™ Streptavidin eFluor™ 660 Conjugate 

Invitrogen™ eBioscience™ Streptavidin eFluor™ 660 Conjugate

Catalog No. 50431780
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50-431-780 25 μg
50-431-782 100 μg
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Contains Sodium azide
Catalog No. 50-431-780 Supplier Invitrogen™ Supplier No. 50431780
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Description

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, immunohistochemistry, and immunocytochemistry applications.

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, immunohistochemistry, and immunocytochemistry applications. Streptavidin binds to biotin with high affinity.

Reported Applications

  • Flow Cytometric Analysis
  • Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections
  • Immunohistochemical Staining of Frozen Tissue Sections
  • Immunocytochemistry
TRUSTED_SUSTAINABILITY

Specifications

Concentration 0.2 mg/mL
Conjugate eFluor 660
Quantity 25 μg
Excitation/Emission 633-647/668 nm
Flow Cytometer Laser Lines Red
Form Liquid
Product Line eBioscience
Product Type Streptavidin Conjugate (fluorescent)
Target Molecule Biotin

Frequently Asked Questions (FAQs)

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.
What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.
What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.
Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

For Research Use Only.

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