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Edvotek™ Advanced Placement Laboratory Kit 4: Plant Pigment Chromatography and Photosynthesis Products for Science Education

Experiment demonstrates light-dependent reactions of photosynthesis by colorimetric assay, and helps students to gain an understanding of the biochemical processes by which they work.

Manufacturer:  Edvotek™ 284

Catalog No. S11284


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Includes: instructions, chloroplast extraction; dilution buffers, reducing reagent, DCIP, standard pigments, chromatography solvent, Sarkosyl, thin layer cellulose chromatography plate, microliter capillary pipets, tubes and transfer pipets.

Requires :
  • Visible wavelength spectrophotometer (see S42669ND)
  • Pencils
  • Clinical benchtop centrifuge
  • Metric rulers
  • Test tube racks
  • Flood light or small portable lamps (60w is sufficient)
  • Pipet pumps or bulbs
  • Distilled water
  • Test tubes, 10mL (13 x 100mm)
  • Beakers, 100mL
  • Pipets, 1 and 5mL
  • Ice
  • Cheesecloth
  • 95-100% isopropanol† (2-propanol)
  • Aluminum foil
  • Fresh spinach


Description

Description

Reformulated solvent systems are less flammable and less toxic than commonly used ether and acetone systems.

  • Gain an understanding of the theory and applications of chromatography
  • Use thin layer chromatography to separate plant pigments carotene, xanthophyll, and chlorophyll
  • Perform an assay of the light reactions of photosynthesis using intact chloroplasts and the dye DCIP

† Rubbing alcohol will not work, as it is only 70% isopropanol.

Order Info

Class size: 10 laboratory groups (40 students).

Specifications

Specifications

40 students
Chloroplast extraction buffer, reducing reagent, chloroplast dilution buffer, dcip, standard pigments, chromatography solvent, sarkosyl, thin layer cellulose chromatography plate, 40 x 5μl capillary pipets, 4 plastic transfer pipets, 24
Refrigerated
Experiment demonstrates light-dependent reactions of photosynthesis by colorimetric assay and helps students to gain an understanding of the biochemical processes by which they work
1.75 hours
Ap™ Biology Lab Kit 4: Plant Pigment Chromatography And Photosynthesis
SDS
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