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Edvotek™ Advanced Placement Laboratory Kit 4: Plant Pigment Chromatography and Photosynthesis 
Experiment demonstrates light-dependent reactions of photosynthesis by colorimetric assay, and helps students to gain an understanding of the biochemical processes by which they work.
Manufacturer: Edvotek™ 284
Includes: instructions, chloroplast extraction; dilution buffers, reducing reagent, DCIP, standard pigments, chromatography solvent, Sarkosyl, thin layer cellulose chromatography plate, microliter capillary pipets, tubes and transfer pipets.
Requires :
- Visible wavelength spectrophotometer (see S42669ND)
- Pencils
- Clinical benchtop centrifuge
- Metric rulers
- Test tube racks
- Flood light or small portable lamps (60w is sufficient)
- Pipet pumps or bulbs
- Distilled water
- Test tubes, 10mL (13 x 100mm)
- Beakers, 100mL
- Pipets, 1 and 5mL
- Ice
- Cheesecloth
- 95-100% isopropanol† (2-propanol)
- Aluminum foil
- Fresh spinach
Description
Reformulated solvent systems are less flammable and less toxic than commonly used ether and acetone systems.
- Gain an understanding of the theory and applications of chromatography
- Use thin layer chromatography to separate plant pigments carotene, xanthophyll, and chlorophyll
- Perform an assay of the light reactions of photosynthesis using intact chloroplasts and the dye DCIP
† Rubbing alcohol will not work, as it is only 70% isopropanol.
Order Info
Class size: 10 laboratory groups (40 students).
Specifications
| 40 students | |
| Chloroplast extraction buffer, reducing reagent, chloroplast dilution buffer, dcip, standard pigments, chromatography solvent, sarkosyl, thin layer cellulose chromatography plate, 40 x 5μl capillary pipets, 4 plastic transfer pipets, 24 | |
| Refrigerated |
| Experiment demonstrates light-dependent reactions of photosynthesis by colorimetric assay and helps students to gain an understanding of the biochemical processes by which they work | |
| 1.75 hours | |
| Ap™ Biology Lab Kit 4: Plant Pigment Chromatography And Photosynthesis |
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