Edvotek™ Advanced Placement Laboratory Kit 4: Plant Pigment Chromatography and Photosynthesis

Description

Includes

instructions, chloroplast extraction; dilution buffers, reducing reagent, DCIP, standard pigments, chromatography solvent, Sarkosyl, thin layer cellulose chromatography plate, microliter capillary pipets, tubes and transfer pipets.

Requires

• Visible wavelength spectrophotometer (see S42669ND)
• Pencils
• Clinical benchtop centrifuge
• Metric rulers
• Test tube racks
• Flood light or small portable lamps (60w is sufficient)
• Pipet pumps or bulbs
• Distilled water
• Test tubes, 10mL (13 x 100mm)
• Beakers, 100mL
• Pipets, 1 and 5mL
• Ice
• Cheesecloth
• 95-100% isopropanol† (2-propanol)
• Aluminum foil
• Fresh spinach

Experiment demonstrates light-dependent reactions of photosynthesis by colorimetric assay, and helps students to gain an understanding of the biochemical processes by which they work.

Reformulated solvent systems are less flammable and less toxic than commonly used ether and acetone systems.

• Gain an understanding of the theory and applications of chromatography
• Use thin layer chromatography to separate plant pigments carotene, xanthophyll, and chlorophyll
• Perform an assay of the light reactions of photosynthesis using intact chloroplasts and the dye DCIP

Order Info

Class size: 10 laboratory groups (40 students).

† Rubbing alcohol will not work, as it is only 70% isopropanol.

Specifications

• Product Type: AP Biology Lab Kit 4: Plant Pigment Chromatography and Photosynthesis
• Grade: 9 to Undergraduate
• For Use With (Application): Experiment demonstrates light-dependent reactions of photosynthesis by colorimetric assay and helps students to gain an understanding of the biochemical processes by which they work
• Includes: Chloroplast extraction buffer, reducing reagent, chloroplast dilution buffer, dcip, standard pigments, chromatography solvent, sarkosyl, thin layer cellulose chromatography plate, 40 x 5μl capillary pipets, 4 plastic transfer pipets, 24
• Time Range: 1.75 hr.
• Content And Storage: Refrigerated
• Class Size: 40 Students