Edvotek™ AIDS Kit II: Simulation of HIV Detection by Western Blot

Description

Includes

instructions, samples, standard molecular weight markers, protein agarose, various buffers and reagents, PVDF membrane, filter paper, stain, 1 ml pipet, 100 ml graduated cylinder

Requires

• Horizontal gel electrophoresis apparatus
• Hotplate, micropipets, incubation oven and shaker platform
• All necessary safety equipment
• Isopropanol, glacial acetic acid and distilled water

Provides a comprehensive introduction to the molecular biology and pathogenesis of AIDS.

• Separate protein samples using horizontal agarose electrophoresis
• Samples are transferred to a membrane and detected by staining
• Demonstrate the concepts and methodology involved with Western blots

Instructor's Guide provides:

• Pre-lab preparations and common pitfalls to avoid
• Answers to study questions and MSDS sheets

Does not contain HIV or its derivatives. No components have been prepared from human sources.

Specifications

• Product Type: Electrophoresis Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Separate protein samples from hypothetical patients on agarose gels, transfer the samples to a membrane and detect the simulated HIV proteins
• Includes: Instructions, samples, standard molecular weight markers, protein agarose, various buffers and reagents, PVDF membrane, filter paper, stain, 1 mL pipet, 100 mL graduated cylinder
• Quantity: 6 Blots
• Requires: Electrophoresis apparatus, power supply, automatic micropipettes with tips, microwave or hot plate, incubation oven, shaker platform, lab glassware, small plastic trays, microtest tubes, pipet pumps or bulbs, metric rulers, distilled water, methanol, glacial acetic acid
• Time Range: Electrophoresis: 45 minutes; Blot: overnight; Detection: 25 minutes
• Content And Storage: Some components require refrigerator storage
• Class Size: 6 Lab Groups