Edvotek™ AP LAB 9: Biotechnology — Restriction Enzyme Analysis of DNA

Description

Includes

Instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 ml (or 0.5 ml) microcentrifuge tubes), UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets.

Requires

Horizontal gel electrophoresis apparatus; DC power supply; automatic micropipets with tips; balance; microwave; hotplate or burner; pipet pump; 250mL flasks or beakers; hot gloves; safety goggles; disposable laboratory gloves; small plastic trays or large weigh boats (for gel destaining); DNA visualization system (white light) and distilled or deionized water

For analysis of Eco RI cleavage patterns of lambda DNA

• This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences
• Bateriophage lambda DNA has a linear structure and six Eco RI recognition sites
• Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield six bands (five distinct bands, two are very close in size) corresponding to the DNA fragments

Order Info

• For six gels
• Takes one hour to complete

Specifications

• Product Type: Biotechnology Kit
• Grade: 9 to 12
• For Use With (Application): Restriction enzyme analysis of dna
• Includes: Instructions, dna samples, ultraspec agarose™ powder, concentrated electrophoresis buffer, flashblue™ dna stain, instastain™ blue cards, practice gel loading solution, 1ml pipet, and microtipped transfer pipets
• Quantity: 8 Gels
• Time Range: 60 min.
• Content And Storage: Refrigerated
• Class Size: 10 Lab Groups