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Edvotek™ Cleavage of Lambda DNA with EcoRI
The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for EcoRI (five distinct sites; two very close in size).
Supplier: Edvotek™ 212
Includes:
- Instructions
- Lambda phage DNA
- Concentrated Restriction Enzyme Reaction Buffer
- EDVOTEK™ Enzyme Grade Water
- Eco RI Dryzymes™ endonuclease
- DNA Standard Marker, Reconstitution Buffers
- UltraSpec-Agarose™
- 10X Gel Loading Solution
- 50X Concentrated Electrophoresis Buffer
- Practice Gel Loading Solution
- FlashBlue™ DNA Stain
- InstaStain™ Blue Cards
- Disposable Pipets
- Microtest Tubes
- Semi-log Graph Paper Template
Requires :
- Electrophoresis apparatus
- Power supply
- Automatic pipet with tips
- Water bath
- Balance
- Microwave or hotplate
- Visualization (white light)
- Lab glassware
- Pipet pumps or bulbs
- Metric rulers
- Floating racks
- Distilled or deionized water
- Ice
Description
- Students digest lambda DNA with EcoRI endonuclease
- Digestion products are analyzed by agarose gel electrophoresis
- Complete in 90 minutes
Order Info
Contains materials for 10 restriction digestions and five gels.
Recommended Storage
Some components require freezer storage
Specifications
Cleavage of Lambda DNA with Eco Ri Endonuclease Kit | |
Instructions, lambda dna, dryzymes™, reconstitution buffer, restriction enzyme reaction buffer, enzyme grade water, standard dna fragments, various solutions and buffers, agarose powder, flashblue™ stain | |
90 min. |
Digestion products are analyzed by agarose gel electrophoresis | |
10 Restriction Digestions and 5 Gels | |
Refrigerated; Store at: -20°C; -4°F |
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