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Edvotek™ Cleavage of Lambda DNA with EcoRI
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Catalog No. S05644 Shop All EDVOTEK Products
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S05644 5 Lab Groups
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Catalog No. S05644 Supplier Edvotek™ Supplier No. 212
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Description

Includes

  • Instructions
  • Lambda phage DNA
  • Concentrated Restriction Enzyme Reaction Buffer
  • EDVOTEK™ Enzyme Grade Water
  • Eco RI Dryzymes™ endonuclease
  • DNA Standard Marker, Reconstitution Buffers
  • UltraSpec-Agarose™
  • 10X Gel Loading Solution
  • 50X Concentrated Electrophoresis Buffer
  • Practice Gel Loading Solution
  • FlashBlue™ DNA Stain
  • InstaStain™ Blue Cards
  • Disposable Pipets
  • Microtest Tubes
  • Semi-log Graph Paper Template

Requires

  • Electrophoresis apparatus
  • Power supply
  • Automatic pipet with tips
  • Water bath
  • Balance
  • Microwave or hotplate
  • Visualization (white light)
  • Lab glassware
  • Pipet pumps or bulbs
  • Metric rulers
  • Floating racks
  • Distilled or deionized water
  • Ice

The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for EcoRI (five distinct sites; two very close in size).

  • Students digest lambda DNA with EcoRI endonuclease
  • Digestion products are analyzed by agarose gel electrophoresis
  • Complete in 90 minutes

Order Info

Contains materials for 10 restriction digestions and five gels.

Recommended Storage

Some components require freezer storage

Specifications

Grade 9 to Undergraduate
For Use With (Application) Digestion products are analyzed by agarose gel electrophoresis
Includes Instructions, lambda dna, dryzymes™, reconstitution buffer, restriction enzyme reaction buffer, enzyme grade water, standard dna fragments, various solutions and buffers, agarose powder, flashblue™ stain
Quantity 10 Restriction Digestions and 5 Gels
Time Range 90 min.
Content And Storage Refrigerated; Store at: -20°C; -4°F
Class Size 5 Lab Groups
Product Type Cleavage of Lambda DNA with Eco Ri Endonuclease Kit
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