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Edvotek™ Cleavage of Lambda DNA with EcoRI
SDPSCI_ED

The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for EcoRI (five distinct sites; two very close in size).

Supplier:  Edvotek™ 212

Catalog No. S05644


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Includes:
  • Instructions
  • Lambda phage DNA
  • Concentrated Restriction Enzyme Reaction Buffer
  • EDVOTEK™ Enzyme Grade Water
  • Eco RI Dryzymes™ endonuclease
  • DNA Standard Marker, Reconstitution Buffers
  • UltraSpec-Agarose™
  • 10X Gel Loading Solution
  • 50X Concentrated Electrophoresis Buffer
  • Practice Gel Loading Solution
  • FlashBlue™ DNA Stain
  • InstaStain™ Blue Cards
  • Disposable Pipets
  • Microtest Tubes
  • Semi-log Graph Paper Template

Requires :
  • Electrophoresis apparatus
  • Power supply
  • Automatic pipet with tips
  • Water bath
  • Balance
  • Microwave or hotplate
  • Visualization (white light)
  • Lab glassware
  • Pipet pumps or bulbs
  • Metric rulers
  • Floating racks
  • Distilled or deionized water
  • Ice


Description

Description

  • Students digest lambda DNA with EcoRI endonuclease
  • Digestion products are analyzed by agarose gel electrophoresis
  • Complete in 90 minutes

Order Info

Contains materials for 10 restriction digestions and five gels.

Recommended Storage

Some components require freezer storage

Specifications

Specifications

Cleavage of Lambda DNA with Eco Ri Endonuclease Kit
Instructions, lambda dna, dryzymes™, reconstitution buffer, restriction enzyme reaction buffer, enzyme grade water, standard dna fragments, various solutions and buffers, agarose powder, flashblue™ stain
90 min.
Digestion products are analyzed by agarose gel electrophoresis
10 Restriction Digestions and 5 Gels
Refrigerated; Store at: -20°C; -4°F
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