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Edvotek™ Clinical Diagnostic Immunoblot
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Catalog No. S96347 Shop All EDVOTEK Products
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10 Lab Groups
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Catalog No. Class Size
S96347 10 Lab Groups
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Catalog No. S96347 Supplier Edvotek™ Supplier No. 276
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Description

Includes

  • Antigen
  • Antibody to antigen
  • IgG-horseradish peroxidase conjugate
  • Peroxidase substrate
  • Hydrogen peroxide
  • Substrate dilution buffer
  • Tris buffered saline (pH 7.5)
  • Instant nonfat dry milk
  • Nylon membranes
  • Petri dishes (60mm diameter)
  • Complete instructions, background information and study questions

Requires

  • Forceps
  • Graph paper
  • Rulers
  • Scissors
  • Shaking platform (optional)
  • Distilled water

Demonstrates a sensitive immunological assay used in research and clinical laboratories to quantify protein antigens.

Clinical diagnostics kits employ the principles of the immunoblot (dot blot), a technique used to determine the presence of an antigen.

  • In this experiment, students will prepare a dot blot membrane to determine the presence of an antigen
  • Antigens are absorbed into a membrane that is then treated sequentially with an antigen-specific antibody solution and a secondary antibody conjugated to an enzyme
  • The enzyme-substrate reaction generates a color product that precipitates on the membrane, indicating a positive reaction

Order Info

Class size: 10 groups.

Specifications

Grade 9 to Undergraduate
For Use With (Application) To determine the presence of an antigen
Includes Instructions, antigen, antibodies, various reagents and buffers, instant nonfat dry milk, hydrogen peroxide, nylon membranes and petri dishes
Time Range 1.5 to 2 hr.
Content And Storage Refrigerated; Store at: 4°C; 39°F
Class Size 10 Lab Groups
Product Type Classroom Kit
Product Title
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