Edvotek™ Clinical Diagnostic Immunoblot

Description

Includes

• Antigen
• Antibody to antigen
• IgG-horseradish peroxidase conjugate
• Peroxidase substrate
• Hydrogen peroxide
• Substrate dilution buffer
• Tris buffered saline (pH 7.5)
• Instant nonfat dry milk
• Nylon membranes
• Petri dishes (60mm diameter)
• Complete instructions, background information and study questions

Requires

• Forceps
• Graph paper
• Rulers
• Scissors
• Shaking platform (optional)
• Distilled water

Demonstrates a sensitive immunological assay used in research and clinical laboratories to quantify protein antigens.

Clinical diagnostics kits employ the principles of the immunoblot (dot blot), a technique used to determine the presence of an antigen.

• In this experiment, students will prepare a dot blot membrane to determine the presence of an antigen
• Antigens are absorbed into a membrane that is then treated sequentially with an antigen-specific antibody solution and a secondary antibody conjugated to an enzyme
• The enzyme-substrate reaction generates a color product that precipitates on the membrane, indicating a positive reaction

Order Info

Class size: 10 groups.

Specifications

• Product Type: Classroom Kit
• Grade: 9 to Undergraduate
• For Use With (Application): To determine the presence of an antigen
• Includes: Instructions, antigen, antibodies, various reagents and buffers, instant nonfat dry milk, hydrogen peroxide, nylon membranes and petri dishes
• Time Range: 1.5 to 2 hr.
• Content And Storage: Refrigerated; Store at: 4°C; 39°F
• Class Size: 10 Lab Groups