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Edvotek™ Diversity of Fish Proteins
SDPSCI_ED

Catalog No. S05650
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Class Size:
6 Lab Groups

Includes

Instructions, fish LyphoProtein™ samples, protein molecular weight standards, practice gel-loading solution, buffer and Protein InstaStain™

Determine the relationship between three fish species based on their protein profiles.

Study the diversity of fish with these prestained, lyophilized proteins.

  • Students extract and prestain total protein from perch, walleye and salmon using an indicator dye
  • After separating each fish protein sample by denaturing SDS-polyacrylamide gel electrophoresis, students find a characteristic banding pattern Pattern can be used to identify the specific species

Experiment times:

  • Electrophoresis, one hour
  • Staining, 20 minutes
  • Destaining, two hours

All You Need

  • 3 polyacrylamide gels (12%)
  • MV10 vertical gel electrophoresis apparatus
  • power supply
  • microcentrifuge
  • hot plate or burner
  • vortex
  • white light visualization system
  • automatic micropipet with fine tips
  • test tube holders
  • lab glassware
  • methanol
  • glacial acetic acid
  • distilled water.

Order Info

For six groups sharing three gels.

Recommended Storage

Some components require freezer storage

Specifications

Product Type Protein Kit
Grade 9 to Undergraduate
For Use With (Application) Identify the specific species
Includes Instructions, fish lyphoprotein™ samples, protein molecular weight standards, practice gel loading solution, buffer, protein instastain™
Time Range 60 minutes; Staining time: 20 minutes
Content And Storage Refrigerated. Some components require freezer storage.
Class Size 6 Lab Groups
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