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Edvotek™ DNA Fingerprinting Using Restriction Enzymes



Description
Includes
instructions, ''crime scene'' and ''suspect'' Ready-to-Load™ DNA samples, DNA Standard Marker, Dryzymes™ - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, Enzyme Grade Water, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, InstaStain™ Blue Cards, FlashBlue™ DNA Stain, Disposable Pipets, & Microcentrifuge Tubes
Requires
Required: Horizontal gel electrophoresis apparatus; DC power supply; Automatic micropipets with tips; Water bathHotplate, Bunsen burner or microwave oven
Recommended: DNA visualization system (white light); Staining tray and net; 250mL flasks; Pipet pump; Hot gloves; Marking pens;Distilled or deionized water; Ice
- The objective of this simulated forensic analysis is to develop an understanding of DNA fingerprinting by use of restriction enzymes
- Hypothetical crime scene samples are provided in addition to DNA samples, restriction enzymes and reaction buffer to digest suspect's DNA
- Digestion products are separated by agarose gel electrophoresis
- Students determine the guilty suspect through identification of the unique restriction enzyme fragmentation patterns
- Does not contain human DNA
- Some components require refrigeration, some require freezing
Order Info
Contains materials for six gels (six lanes each) with InstaStain™ Methylene Blue.
Specifications
Specifications
Product Type | DNA Fingerprinting Using Restriction Enzymes Classroom Kit |
Grade | 9 to Undergraduate |
For Use With (Application) | For dna fingerprinting |
Quantity | 6 Gels |
Requires | Electrophoresis apparatus; power supply; automatic pipet with tips; waterbath; balance; microwave or hot plate; visualization (white light); misc. labware; pipet pumps or bulbs; metric rulers; floating racks; photodocumentation system (optional) |
Time Range | 1.45 hr. |
Content And Storage | Some components require refrigeration and some require freezing |
Class Size | 8 Lab Groups |
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