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Edvotek™ Exploring Biotechnology with Green Fluorescent Protein (GFP)
SDPSCI_ED

Catalog No. S05657 Shop All EDVOTEK Products
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Class Size:
10 Lab Groups

Description

Includes

Instructions; BactoBeads; Plasmid DNA for GFP; IPTG; Ampicillin antibiotic; Calcium chloride; ReadyPour™ luria broth agar; Luria broth media for recovery; Petri plates; Pipets; Calibrated transfer pipets; Inoculating loops; Microtest tubes with attached caps; Toothpicks; Dry matrix for columns; Chromatography columns; Green fluorescent protein extracts; Elution buffer; Protein molecular weight standards; Protein denaturation solution; Glycerol solution; Tris-Glycine-SDS buffer; Stains

Requires

Incubation oven; Two water baths; Microwave or hotplate; Automatic micropipet and tips; Pipet pumps or bulbs; Ice; Long-wave UV light; Ring stand and clamps; Lab glassware; Ice; Vertical gel electrophoresis apparatus; Power supply; Three polyacrylamide gels (12%); Plastic trays or large weigh boats for optional staining and destaining; Glacial acetic acid; Methanol

Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP).

  • Bacterial cells are transformed to express the green fluorescent protein (GFP)
  • Transformed cells are then grown and the GFP is purified by column chromatography
  • The purity of the protein fractions are analyzed by SDS polyacrylamide electrophoresis

Experiment times:

  • Module I: Transformation, 45 minutes
  • Module II: Isolation of GFP, 45 minutes
  • Module III: Purification of GFP by Chromatography, 45 minutes
  • Module IV: Analysis by GFP by Denaturing SDS Gel Electrophoresis, one hour

Order Info

Contains materials for six experiments with four modules each.

Recommended Storage

Some components requre freezer storage

Specifications

Grade 9 to Undergraduate
For Use With (Application) Provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (gfp)
Quantity 10 experiments
Time Range 3 hours 15 minutes; Staining time: 30 to 120 minutes
Content And Storage Refrigerated. Some components requre freezer storage.
Class Size 10 Lab Groups
Product Type Green Fluorescent Protein Kit
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