Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Education Supplies
  4. Biotechnology Classroom Products
  5. Biotechnology Classroom Supplies
  6. S05657

Edvotek™ Exploring Biotechnology with Green Fluorescent Protein (GFP)
SDPSCI_ED

Catalog No. S05657 Shop All EDVOTEK Products
Change view
Click to view available options
Class Size:
10 Lab Groups
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Class Size
S05657 10 Lab Groups
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. S05657 Supplier Edvotek™ Supplier No. 303
Only null left
Add to Cart
Add to Cart

Description

Includes

Instructions; BactoBeads; Plasmid DNA for GFP; IPTG; Ampicillin antibiotic; Calcium chloride; ReadyPour™ luria broth agar; Luria broth media for recovery; Petri plates; Pipets; Calibrated transfer pipets; Inoculating loops; Microtest tubes with attached caps; Toothpicks; Dry matrix for columns; Chromatography columns; Green fluorescent protein extracts; Elution buffer; Protein molecular weight standards; Protein denaturation solution; Glycerol solution; Tris-Glycine-SDS buffer; Stains

Requires

Incubation oven; Two water baths; Microwave or hotplate; Automatic micropipet and tips; Pipet pumps or bulbs; Ice; Long-wave UV light; Ring stand and clamps; Lab glassware; Ice; Vertical gel electrophoresis apparatus; Power supply; Three polyacrylamide gels (12%); Plastic trays or large weigh boats for optional staining and destaining; Glacial acetic acid; Methanol

Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP).

  • Bacterial cells are transformed to express the green fluorescent protein (GFP)
  • Transformed cells are then grown and the GFP is purified by column chromatography
  • The purity of the protein fractions are analyzed by SDS polyacrylamide electrophoresis

Experiment times:

  • Module I: Transformation, 45 minutes
  • Module II: Isolation of GFP, 45 minutes
  • Module III: Purification of GFP by Chromatography, 45 minutes
  • Module IV: Analysis by GFP by Denaturing SDS Gel Electrophoresis, one hour

Order Info

Contains materials for six experiments with four modules each.

Recommended Storage

Some components requre freezer storage

Specifications

Grade 9 to Undergraduate
For Use With (Application) Provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (gfp)
Quantity 10 experiments
Time Range 3 hours 15 minutes; Staining time: 30 to 120 minutes
Content And Storage Refrigerated. Some components requre freezer storage.
Class Size 10 Lab Groups
Product Type Green Fluorescent Protein Kit
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.