Edvotek™ In Search of the Sickle Cell Gene by Southern Blot

Description

Includes

instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain

Requires

• Horizontal gel electrophoresis apparatus
• Hotplate
• Micropipets
• Balance
• Water bath
• Incubation oven
• 250mL Flasks
• All necessary safety equipment
• Concentrated HCl
• NaOH
• Forceps Distilled or deionized water

Use Southern blots to detect mutations while gaining insight about the effects of sickle cell anemia.

Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.

Specifications

• Product Type: Electrophoresis Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Southern blotting
• Includes: Instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain
• Requires: Electrophoresis apparatus, power supply, microwave or hot plate, water bath and 80°C incubation oven
• Time Range: Electrophoresis: 45 min.; Blot: overnight; Stain and Destain: 10 min.
• Content And Storage: Some components require freezer storage
• Class Size: 5 Lab Groups