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Description
Includes
- Shoot initiation growth medium; shoot elongation growth medium
- Tween™
- Sterile Petri plates
- Parafilm
- Growth container
- Peat pellets
- 10mL pipets
- Complete instructions, background information and study questions
Requires
- Saintpaulia ionantha (African Violet)
- Distilled water
- 70% and 95% ethanol
- 100% isopropanol
- Bleach
- Forceps
- Scalpels and blades
- Laminar flow hood or sterile transfer hood
- Antibacterial soap
- Gloves
- Fluorescent light source
- Beaker, 600mL
- Sterile beakers
- Aluminum foil
- Pipet pump
- 10cm test tubes and racks
- Spray bottle
- Two-part experiment establishes cell cultures from plant materials and focuses on plant regeneration
- Learn about sterile testing procedures, components of plant culture media and effects of plant growth regulators on cell cultures
Specifications
Specifications
| Grade | 9 to Undergraduate |
| For Use With (Application) | Students will establish cell cultures of african violets from leaves |
| Includes | Shoot initiation and elongation growth medium, tween™, sterile petri plates, parafilm, growth containers, peat pellets, 10ml pipets, complete instructions, background information and study questions |
| Requires | Healthy african violet (Saintpaulia ionantha), microwave or hot plate, distilled water, 70% and 95% ethanol, 100% isopropanol, bleach, forceps, scalpels and blades, laminar flow hood or sterile transfer hood, antibacterial soap, gloves, fluorescent light |
| Time Range | 60 minutes and several weeks for growth |
| Content And Storage | Refrigerated |
| Class Size | 10 Lab Groups |
| Product Type | Introduction to Plant Cell Culture |
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