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  6. S05656

Edvotek™ Purification of the Restriction Enzyme Eco RI
SDPSCI_ED

Catalog No. S05656
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Class Size:
5 Lab Groups

Description

Includes

  • Instructions
  • Ion exchange matrix
  • Chromatography columns
  • E. coli cell extract
  • Equilibration and elution buffer
  • Lambda DNA
  • Lambda/Eco RI Marker
  • KCl
  • Glycerol
  • Dilution and reaction buffers
  • Gel loading solution
  • Agarose
  • Electrophoresis buffer
  • Stain

In this Edvotek experiment, students actually purify the restriction enzyme, Eco RI.

In this experiment, students actually purify the restriction enzyme, Eco RI. This procedure utilizes an ion exchange chromatography step for Eco RI purification.

  • Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis
  • Fractions that contain Eco RI are identified and pooled
  • Total and specific activities are calculated
  • Recommended for college level courses

Experiment times:

  • Packing column, 45 minutes
  • Restriction analysis A, 35 minutes
  • Restriction analysis B, 50 minutes
  • Gel Prep, 30 minutes
  • Electrophoresis, 30 minutes
  • Staining and destaining, two minutes

All You Need

  • horizontal gel electrophoresis apparatus
  • power supply
  • UV visualization system
  • waterbath
  • microcentrifuge
  • microwave or hot plate
  • UV spectrophotometer & cuvettes
  • automatic micropipet with tips
  • ring stands & clamps
  • 10 ml pipets
  • lab glassware
  • ice and ice buckets.

Order Info

For five purifications

Recommended Storage

Some components requre freezer storage

Specifications

Product Type Purification Kit
Grade 9 to Undergraduate
For Use With (Application) Purify the restriction enzyme, eco ri
Includes Instructions, ion exchange matrix, chromatography columns, E. coli cell extract, equilibration and elution Buffer, Lambda DNA, Lambda or Eco RI Marker, KCl, glycerol, dilution and reaction Buffers, gel loading solution, Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide
Quantity 5 purifications
Time Range 3 hours 15 minutes
Content And Storage Refrigerated. Some components require freezer storage.
Class Size 5 Lab Groups
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