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Edvotek™ Purification of the Restriction Enzyme Eco RI

In this Edvotek experiment, students actually purify the restriction enzyme, Eco RI.

Supplier: Edvotek™ 302

Catalog No. S05656

$327.00 / Each of 1

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Includes:

Instructions
Ion exchange matrix
Chromatography columns
E. coli cell extract
Equilibration and elution buffer
Lambda DNA
Lambda/Eco RI Marker
KCl
Glycerol
Dilution and reaction buffers
Gel loading solution
Agarose
Electrophoresis buffer
Stain

Description

In this experiment, students actually purify the restriction enzyme, Eco RI. This procedure utilizes an ion exchange chromatography step for Eco RI purification.

Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis
Fractions that contain Eco RI are identified and pooled
Total and specific activities are calculated
Recommended for college level courses

Experiment times:

Packing column, 45 minutes
Restriction analysis A, 35 minutes
Restriction analysis B, 50 minutes
Gel Prep, 30 minutes
Electrophoresis, 30 minutes
Staining and destaining, two minutes

All You Need

horizontal gel electrophoresis apparatus
power supply
UV visualization system
waterbath
microcentrifuge
microwave or hot plate
UV spectrophotometer & cuvettes
automatic micropipet with tips
ring stands & clamps
10 ml pipets
lab glassware
ice and ice buckets.

Order Info

For five purifications

Recommended Storage

Some components requre freezer storage

Specifications

Product Type	Purification Kit
Includes	Instructions, ion exchange matrix, chromatography columns, E. coli cell extract, equilibration and elution Buffer, Lambda DNA, Lambda or Eco RI Marker, KCl, glycerol, dilution and reaction Buffers, gel loading solution, Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide
Time Range	3 hours 15 minutes

For Use With (Application)	Purify the restriction enzyme, eco ri
Quantity	5 purifications
Content And Storage	Refrigerated. Some components require freezer storage.

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