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Edvotek™ Sickle Cell Gene Detection
SCI_ED

Gain an understanding of the effect of mutations on health and disease.

Supplier:  Edvotek™ 116

Catalog No. S94670


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Includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets

Requires : Horizontal gel electrophoresis apparatus; D.C. power supply; Automatic micropipets with tips; Balance; Microwave, hotplate or burner; Pipet pump; 250mL flasks or beakers; Hot gloves; Safety goggles and disposable laboratory gloves; Small plastic trays or large weigh boats (for gel destaining); DNA visualization system (white light); Distilled or deionized water

Description

Description

  • Genetically inherited diseases in humans can be caused by a single nucleotide mutation resulting in a change. Such a substitution can affect the structure and function of a critical protein, rendering it partially or completely nonfunctional. A classic example is hemoglobin in sickle cell anemia
  • Agarose gel-based experiment designed for DNA staining and visualization with InstaStain™ Methylene Blue
  • Demonstrates the use of a restriction enzyme that discriminates between the normal and sickle cell hemoglobin genes
  • This experiment does not contain human DNA
Specifications

Specifications

Sickle Cell Gene Detection (DNA-Based) Classroom Kit
Instructions; ready-to-load™ quickstrip™ dna samples; ultraspec agarose™ powder; practice gel loading solution; electrophoresis buffer; instastain™ blue and flashblue™
Electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system
Refrigerated
To investigate the restriction enzyme that discriminates between hba (normal) and hbs (disease) genes and perform a simulated test on a patient
8 Gels
45 min.
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