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Edvotek™ Sickle Cell Gene Detection



Description
Includes
Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets
Requires
Horizontal gel electrophoresis apparatus; D.C. power supply; Automatic micropipets with tips; Balance; Microwave, hotplate or burner; Pipet pump; 250mL flasks or beakers; Hot gloves; Safety goggles and disposable laboratory gloves; Small plastic trays or large weigh boats (for gel destaining); DNA visualization system (white light); Distilled or deionized water
- Genetically inherited diseases in humans can be caused by a single nucleotide mutation resulting in a change. Such a substitution can affect the structure and function of a critical protein, rendering it partially or completely nonfunctional. A classic example is hemoglobin in sickle cell anemia
- Agarose gel-based experiment designed for DNA staining and visualization with InstaStain™ Methylene Blue
- Demonstrates the use of a restriction enzyme that discriminates between the normal and sickle cell hemoglobin genes
- This experiment does not contain human DNA
Specifications
Specifications
Product Type | Sickle-cell Gene Detection (DNA-based) Classroom Kit |
Grade | 9 to Undergraduate |
For Use With (Application) | To investigate the restriction enzyme that discriminates between hba (normal) and hbs (disease) genes and perform a simulated test on a patient |
Includes | Instructions; ready-to-load™ quickstrip™ dna samples; ultraspec agarose™ powder; practice gel loading solution; electrophoresis buffer; instastain™ blue and flashblue™ |
Quantity | 8 Gels |
Requires | Electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system |
Time Range | 45 min. |
Content And Storage | Refrigerated |
Class Size | 8 Lab Groups |
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