Edvotek™ Sickle Cell Gene Detection

Description

Includes

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets

Requires

Horizontal gel electrophoresis apparatus; D.C. power supply; Automatic micropipets with tips; Balance; Microwave, hotplate or burner; Pipet pump; 250mL flasks or beakers; Hot gloves; Safety goggles and disposable laboratory gloves; Small plastic trays or large weigh boats (for gel destaining); DNA visualization system (white light); Distilled or deionized water

Gain an understanding of the effect of mutations on health and disease.

• Genetically inherited diseases in humans can be caused by a single nucleotide mutation resulting in a change. Such a substitution can affect the structure and function of a critical protein, rendering it partially or completely nonfunctional. A classic example is hemoglobin in sickle cell anemia
• Agarose gel-based experiment designed for DNA staining and visualization with InstaStain™ Methylene Blue
• Demonstrates the use of a restriction enzyme that discriminates between the normal and sickle cell hemoglobin genes
• This experiment does not contain human DNA

Specifications

• Product Type: Sickle-cell Gene Detection (DNA-based) Classroom Kit
• Grade: 9 to Undergraduate
• For Use With (Application): To investigate the restriction enzyme that discriminates between hba (normal) and hbs (disease) genes and perform a simulated test on a patient
• Includes: Instructions; ready-to-load™ quickstrip™ dna samples; ultraspec agarose™ powder; practice gel loading solution; electrophoresis buffer; instastain™ blue and flashblue™
• Quantity: 8 Gels
• Requires: Electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system
• Time Range: 45 min.
• Content And Storage: Refrigerated
• Class Size: 8 Lab Groups