Edvotek™ Transformation of E. coli with Green Fluorescent Protein (GFP)

Description

Includes

instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes

GFP gene was isolated and cloned from the jellyfish Aequorea victoria.

In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.

Order Info

Contains materials for 10 transformations and controls.

Specifications

• Product Type: Biotechnology Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Transformation of e. coli with a plasmid containing the gene for the green fluorescent protein (gfp)
• Includes: Instructions, lyphocells, e. coli gfp host, supercoiled pfluorogreen™, ampicillin, iptg, cacl2, readypour™ luria broth agar (sterile), luria broth medium for recovery (sterile), petri plates (small), petri plates (large
• Time Range: 15 min.
• Content And Storage: Refrigerated; Store at: -20°C; -4°F
• Class Size: 10 Lab Groups