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Edvotek™ Transformation of E. coli with Green Fluorescent Protein (GFP) Products for Science Education

GFP gene was isolated and cloned from the jellyfish Aequorea victoria.

Manufacturer:  Edvotek™ 223/AP08

Catalog No. S68654

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Includes: instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes



In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.

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Contains materials for 10 transformations and controls.



10 lab groups
Instructions, lyphocells, e. coli gfp host, supercoiled pfluorogreen™, ampicillin, iptg, cacl2, readypour™ luria broth agar (sterile), luria broth medium for recovery (sterile), petri plates (small), petri plates (large
Refrigerated; Store at: -20°C; -4°F
Transformation of e. coli with a plasmid containing the gene for the green fluorescent protein (gfp)
Biotechnology Kit
15 minutes
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