Edvotek™ Transformation of E. coli with pGAL™ (Blue Colony)

Description

Includes

BactoBeads, plasmid DNA, buffers, reagents and antibiotic; ReadyPour™ Luria Broth agar; Luria Broth media for recovery; 40 Petri plates; 20 plastic microtipped transfer pipets; Three wrapped 10mL pipets and 25 wrapped 1mL pipets; 20 inoculating loops; 40 microtest tubes with attached lids; Complete instructions, background information and study questions

Requires

Automatic micropipet (5 to 50μL) or 25μL fixed-volume micropipet and tips; Two water baths; Incubation oven; Pipet pumps or bulbs; Ice; Bunsen burner, hotplate or microwave oven; Hot gloves

Presence of IPTG is not required.

In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.

Order Info

Contains materials for 10 transformations.

Specifications

• Product Type: Transformation Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Develop an understanding of bacterial transformation by plasmid dna
• Time Range: 50 min.
• Content And Storage: Some components require refrigeration or freezing
• Class Size: 10 Lab Groups