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Edvotek™ Water Quality Testing III: Multiplexed PCR for Testing Water Bacteria
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Catalog No. S95132 Shop All EDVOTEK Products
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Water Quality Testing II
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S95132 Water Quality Testing II
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Catalog No. S95132 Supplier Edvotek™ Supplier No. 953
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Description

Includes

instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR™ Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes

Requires

Thermal cycler; waterbath, electrophoresis tank; power supply; micropipets to measure between 5 and 50μL; tips and microcentrifuge

Students will investigate presence of contaminants in a water sample.

  • Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required to determine whether harmful microbes are present
  • Students use PCR in multiplex format
  • Single PCR test detects presence of three separate, classroom-safe organisms
  • Analyzed on agarose gels

Experiment times:

  • Setup: 60 minutes
  • PCR: 120 minutes or overnight
  • Electrophoresis: 45 minutes

Specifications

For Use With (Application) To investigate presence of contaminants in a water sample
For Use With (Equipment) Multiplex PCR
Sufficient For 25 Students
Type Water Quality Testing II
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