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Edvotek™ Water Quality Testing III: Multiplexed PCR for Testing Water Bacteria
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Water Quality Testing II
Description
Includes
instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR™ Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes
Requires
Thermal cycler; waterbath, electrophoresis tank; power supply; micropipets to measure between 5 and 50μL; tips and microcentrifuge
- Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required to determine whether harmful microbes are present
- Students use PCR in multiplex format
- Single PCR test detects presence of three separate, classroom-safe organisms
- Analyzed on agarose gels
Experiment times:
- Setup: 60 minutes
- PCR: 120 minutes or overnight
- Electrophoresis: 45 minutes
Specifications
Specifications
| For Use With (Application) | To investigate presence of contaminants in a water sample |
| For Use With (Equipment) | Multiplex PCR |
| Sufficient For | 25 Students |
| Type | Water Quality Testing II |
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