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Edvotek™ Western Blot Analysis, Polyacrylamide-Based
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Catalog No. S90257 Shop All EDVOTEK Products
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6 Lab Groups
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S90257 6 Lab Groups
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Catalog No. S90257 Supplier Edvotek™ Supplier No. 317
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Description

Includes

  • Instructions
  • Negative control
  • All samples and antibodies
  • Various reagents and buffers
  • Membrane and filter paper

Requires

  • Three polyacrylamide gels (12%)
  • Vertical gel electrophoresis apparatus
  • Power supply
  • Automatic micropipet with fine tips
  • Laboratory glassware
  • Metric rulers
  • Distilled or deionized water
  • Glacial acetic acid
  • Methanol

In Western Blot analysis, protein identification is based on antibody and antigen reactions.

  • Proteins are separated on a polyacrylamide gel and are transferred (blotted) to a nylon membrane
  • Membrane is exposed to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme
  • Membrane is then soaked in a substrate solution to develop the color reaction to identify the antigen protein band
  • Molecular weights of the visible bands are measured using prestained protein markers of known molecular weight

Experiment times:

  • Electrophoresis, one hour
  • Blot, overnight
  • Detection, two hours and 30 minutes

Kit does not require an electrotransfer apparatus.

Order Info

Contains materials for six blots.

Specifications

Grade 9 to Undergraduate
For Use With (Application) Proteins are separated on polyacrylamide gels and are transferred (blotted) to a nylon membrane
Includes Instructions, negative control, all samples and antibodies, various reagents and buffers, membrane and filter paper
Quantity 6 blots
Time Range 2.5 hr.
Content And Storage Refrigerated
Class Size 6 Lab Groups
Product Type Western Blot Kit
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