Edvotek™ Western Blot Analysis, Polyacrylamide-Based

Description

Includes

• Instructions
• Negative control
• All samples and antibodies
• Various reagents and buffers
• Membrane and filter paper

Requires

• Three polyacrylamide gels (12%)
• Vertical gel electrophoresis apparatus
• Power supply
• Automatic micropipet with fine tips
• Laboratory glassware
• Metric rulers
• Distilled or deionized water
• Glacial acetic acid
• Methanol

In Western Blot analysis, protein identification is based on antibody and antigen reactions.

• Proteins are separated on a polyacrylamide gel and are transferred (blotted) to a nylon membrane
• Membrane is exposed to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme
• Membrane is then soaked in a substrate solution to develop the color reaction to identify the antigen protein band
• Molecular weights of the visible bands are measured using prestained protein markers of known molecular weight

Experiment times:

• Electrophoresis, one hour
• Blot, overnight
• Detection, two hours and 30 minutes

Kit does not require an electrotransfer apparatus.

Order Info

Contains materials for six blots.

Specifications

• Product Type: Western Blot Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Proteins are separated on polyacrylamide gels and are transferred (blotted) to a nylon membrane
• Includes: Instructions, negative control, all samples and antibodies, various reagents and buffers, membrane and filter paper
• Quantity: 6 blots
• Time Range: 2.5 hr.
• Content And Storage: Refrigerated
• Class Size: 6 Lab Groups