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BIOHIPPO EF1a-H3.3-K27M-Flag/BSD
Supplier: BIOHIPPO BHV194001074
EF1a-H3 3-K27M-Flag/BSD lentiviral vector expressing Flag under EF1a promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human H3 3 K27M ORF cDNA lentivirus Lentiviral particles packaged with expression vector for K27M mutant of human H3 3 histone A (also known as H3 3 or H3-3A) transcript variant 1 An EF1 promoter drives expression of H3 3 K27M fused to a C-terminal V5 tag and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP) or luminescent (firefly luc) reporters
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