Gibco™ Efficient-Pro™ AGT™ Medium

Catalog No.	Quantity
A5322302	10 L
A5322301	1 L
A5322303	100 L
A5322304	500 L

Catalog No. A5322302 Supplier Gibco™ Supplier No. A5322302

Description

Gibco™ Efficient-Pro™ Media delivers superior product performance, improved product titer, better protein quality and lot-to-lot consistency for more control and predictability. Chemically defined and completely scalable, available in Gibco™ AGT™ formats for sustained cell viability.

• A next generation CHO media used in the production of monoclonal antibodies
• AGT Medium offers significant performance and ease-of-use improvements not seen in existing Gibco™ or competitor products
• Proprietary AGT™ format is the only one of its kind in the industry and is the most advanced media format available in the market
• Formulated as a chemically-defined, animal-origin free cell culture media used in the bioproduction of mAbs & other therapeutics in traditional batch & fed-batch bioprocessing workflows
• Free of growth factors and insulin to ensure batch-to-batch consistency and production efficiency
• Designed to work optimally with Efficient-Pro Feed 1 and Efficient-Pro Feed 2 for use in CHO-K1 and DG-44/CHO-S cell lines respectively
• The Efficient-Pro system increases titer and specific productivity, improves protein quality and metabolite profiles
• System is easier to use with less solubility issues than other CHO media and feed offerings
• Increase ease-of-use with Gibco’s™ trademarked, patented Advanced Granulation Technology™ (AGT™) for auto-pH and simplified dissolution
• Sustain cell viability for longer bioreactor runs & maximized titer
• Demonstrated to improve titers in a CHO-K1 cell line up to 147% when paired with Efficient-Pro™ Feed 1 compared to an industry-leader’s media and feed system
• Improve downstream outputs such as protein glycosylation, charge-variants & aggregation
• Speed-up process development and streamline transfer to manufacturing scale
• Increase flexibility with a range of format & packaging configurations
• Decrease cell shock with simplified adaptation protocols

Specifications

• Cell Line: CHO-DG44, CHO-S, CHO-K1, GS-CHO
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Form: Powder (AGT)
• Quantity: 10 L
• Shipping Condition: Room temperature
• Green Features: Sustainable packaging
• Content And Storage: Store in the dark at 2–8°C.
  
  
• Culture Type: Mammalian Cell Culture

Frequently Asked Questions (FAQs)

How should I reconstitute Efficient-Pro AGT Medium (Cat. No. A5322301, A5322302, A5322303, A5322304)?

Please follow the steps shown below to reconstitute Efficient-Pro AGT Medium (Cat. No. A5322301, A5322302, A5322303, A5322304) and to make Complete Medium:

- Measure 90% of the final volume of deionized or distilled water at room temperature (15-30 degrees C).
- Add Efficient-Pro AGT Medium at 27.3 grams/L to water.
- Mix for a minimum of 20 min.
- Use a calibrated vessel to dilute to final production volume with ambient deionized or distilled water.
- Mix for an additional 20 min.
- Measure the pH, then measure and record osmolality.
- Sterilize the product immediately by membrane filtration(positive pressure recommended).
Note: Once the product is filtered, use immediately or store at 2-8 degrees C for up to 6 months. Protect from light.

To make Complete Medium,
- Supplement Efficient-Pro AGT Medium with L‑glutamine or GlutaMAX Supplement at 2-8 mM final concentration prior to use (except for CHO-K1 GS cell lines).
Note: The recommended L‑glutamine and/or GlutaMAX Supplement concentration is 6 mM.
- Add 10 mL/L of HT Supplement for use in applications not requiring DHFR amplification.
- Glucose supplementation may be required for terminal batch cultures and should be determined empirically.
- Add Anti-Clumping Agent (1 mL/L) to the medium to reduce cell aggregation, if required.
Note: Consider reducing L‑glutamine concentration for fed-batch or perfusion protocols, or if the cell line in-use is sensitive to ammonia. Addition of a surfactant such as Pluronic™ F-68 is not required.

What is the shelf-life of Efficient-Pro AGT Medium?

The shelf-life of Efficient-Pro AGT Medium is 12 months from the date of manufacture. The expiration date is provided on the product label.

If my cell line is sensitive to ammonia, can I still use Efficient-Pro AGT Medium?

Yes, however, we recommend reducing the L-glutamine concentration if a decrease in cell viability is observed. The recommended glutamine concentration is 2-8 mM. Another way to reduced ammonia production is to use GlutaMAX Supplement instead of L-glutamine. Addition of Pluronic F-68 is not required.

Does Efficient-Pro AGT Medium require supplementation for use?

When using a CHO cell line with a selection system other than the glutamine synthetase (GS) system, we recommend adding 2-8 mM L-glutamine or GlutaMAX Supplement to Efficient-Pro AGT Medium. Additionally, we recommend making sure that glucose levels do not fall below 2 g/L in culture by supplementing with glucose as needed. Also, if clumping becomes an issue, then add anti-clumping agent (ACA) to your cultures at 1% v/v.

What is the concentration of glucose in Efficient Pro-AGT Medium?

Efficient Pro-AGT Medium contains 4.7 g/L of glucose.

Can CHO cells that are created or frozen in other production media be thawed into Efficient-Pro AGT Medium or adapted for growth and production in Efficient-Pro AGT Medium?

Cells that are grown in or adapted to, and frozen in other cell culture media readily adapt to Efficient-Pro AGT Medium. However, if your cells are experiencing a significant lag or delay in growth, please directly adapt the cells into Efficient-Pro AGT Medium and passage them for a minimum of 3 passages, until a consistent doubling time is observed.

For research use only. Not for use in diagnostic procedures.