MilliporeSigma™ EGF-Stimulated A431 Cell Lysate
For use in Western Blotting
Manufacturer: MilliporeSigma™ 12302
EGF-Stimulated A431 Cell Lysate in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1mg/mL aprotinin, 1mg/mL leupeptin, 1mg/mL pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue). Routinely evaluated as a positive control for many of Upstates antibodies.
|6 months at -20°, C|
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