Invitrogen™ Human EGFR (ErbB1) [L858R], GST Tag Recombinant Protein

Catalog No.	Quantity
PV4128	10 μg

Catalog No. PV4128 Supplier Invitrogen™ Supplier No. PV4128

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Cytoplasmic.

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Specifications

• Accession Number: P00533
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 0.5mM EDTA, 50% glycerol, 0.02% Triton X-100, 2mM DTT, 150mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 1956
• Molecular Weight (g/mol): 90.5 kDa
• Name: Human EGFR (ErbB1) [L858R], GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: EGFR; Erbb1
• Common Name: EGFR (L858R Mutant)
• Gene Symbol: EGFR
• Kinase Family: EGF Receptor (ErbB) Kinase Family
• Protein Length: aa 668-1210: L858R
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 668-1210: L858R
• Content And Storage: Storage Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X-100, 2 mM DTT, 50% Glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic, Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Zin-LYTE™, Omnia™ Bulk Substrate
• Target Entry: EGFR L858R
• Target Gene: Epidermal growth factor receptor
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic Domain, Cytoplasmic
• Form: Liquid
• Protein Subtype: Receptor Tyrosine Kinases
• Research Category: Drug Discovery: Oncology, Signal Transduction
• Purity or Quality Grade: 60% by SDS-PAGE