Invitrogen™ Human EGFR (ErbB1) [L861Q], GST Tag Recombinant Protein

Catalog No.	Quantity
PV4191	1 mg
PR7435A	100 μg

Catalog No. PV4191 Supplier Invitrogen™ Supplier No. PV4191

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots; aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Cytoplasmic

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Specifications

• Accession Number: NP_005219.2; L861Q
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.5mM EDTA, 50% glycerol, 0.02% Triton X-100, 150mM NaCl, 2mM DTT and no preservative; pH 7.5
• Molecular Weight (g/mol): 90.5 kDa
• Name: Human EGFR (ErbB1) [L861Q], GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: EGFR (ErbB1) L861Q
• Common Name: EGFR (L861Q Mutant)
• Kinase Family: EGF Receptor (ErbB) Kinase Family
• Protein Length: 668-1210: L858R, 668-1210; L861Q
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 668-1210: L858R, 668-1210; L861Q
• Content And Storage: Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X-100, 2 mM DTT, 50% glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Form: Cytoplasmic
• Form: Liquid
• Protein Subtype: EGF (Epidermal Growth Factor)
• Research Category: Drug Development