ACROBiosystems Recombinant Protein;Biotinylated Human EGFR, His Tag & Avi Tag (Avitag™);HEK293;200UG;EGR,EGFR,EGF R,ERBB,ERBB1,HER1,PIG61,mENA,EGF-R,ERBB-1,HER-1,EGFRvIII

Supplier:  ACROBiosystems EGRH82E3200UG

Recombinant Protein;Biotinylated Human EGFR, His Tag & Avi Tag (Avitag™);200UG;Biotinylated Human EGF R, His Tag, Avi Tag (EGR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 72.3 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag (Cat. No. EGR-H82E3) with a linear range of 2-6 ng/mL (QC tested).;Immobilized Human EGF, Tag Free (Cat. No. EGF-H4114) at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag (Cat. No. EGR-H82E3) with a linear range of 0.156-5 μg/mL (Routinely tested).;Cells were transfected with anti-EGFR scFv and cultured for 3 days. Three days post-transfection, 1e6 cells were stained for anti-EGFR scFv with Biot

Catalog No. 50-165-8297