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AURORA BIOLABS LLC eIF4E/eIF4G Binding Assay, 384 rxn
Supplier: AURORA BIOLABS LLC 34343BK384
A fluorescence-labelled tracer and the N-terminal tagged full-length human eIF4E/eIF4G complex are used in this assay kit. A Terbium-labeled antibody binding to the tag on eIF4E serves as a fluorescence donor (HTRF donor). The binding of the fluorescence-labeled tracer to the eIF4E brings Terbium on the anti-Tag antibody close to the fluorophore on the tracer (HTRF acceptor). Activation of the Terbium results in fluorescence resonance energy transfer (FRET). Thus, the binding status can be quantitively measured by calculating the ratio of the emission fluorescence intensity of the acceptor (665 nm) and donor (620 nm). The competitive binding of a non-fluorescence compound will reduce the FRET signal.
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