Invitrogen™ Alanine Aminotransferase (ALT/GPT) Activity Kit

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Alanine Aminotransferase (ALT/GPT) Activity Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ALT activity in animal serum, plasma, tissue, cultured cells, and cell culture supernatant. ALT is an important pyridoxal phosphate dependent enzyme in the intermediate metabolism of glucose and proteins. Clinically, the activity of serum alanine aminotransferase is often used as a marker for alcoholic liver disease, liver cirrhosis, and acute viral hepatitis.

In this assay, ALT catalyzes the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Phenylhydrazine is added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is a reddish brown solution under alkaline conditions, and ALT activity can be calculated by measuring the OD values at 510 nm.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.75–72.3 IU/L
• Assay Sensitivity: 0.75 IU/L
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates
• For Use With (Equipment): Microplate Reader
• Incubation Time: 50 min.
• Interassay CV: 9
• Intraassay CV: 5.3
• Quantity: 96 tests
• Sample Volume: 10 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Alanine Aminotransferase (ALT/GPT)
• Total Assay Time: 80 min.