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Invitrogen™ Alkaline Phosphatase (ALP) Activity Kit

Catalog No. EEA002
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EEA002 96 tests
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Catalog No. EEA002 Supplier Invitrogen™ Supplier No. EEA002
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Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Alkaline Phosphatase (ALP) Activity Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ALP activity in various sample types. ALP is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase where each catalytic site contains three metal ions.

The assay is based on the ability of alkaline phosphatase to decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol reacts with 4-aminopyrine in alkaline solution and oxidizes with potassium ferricyanide to form a red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 0.13–50 King Units/100 mL
Assay Sensitivity 0.13 King Units/100 mL
Content And Storage Store at 2–8°C.
Detection Method Colorimetric
Sample Type Plasma, Serum, Tissue Homogenates, Whole Cells
For Use With (Equipment) Microplate Reader
Incubation Time 15 min.
Interassay CV 8.5
Intraassay CV 5.1
Quantity 96 tests
Sample Volume 5 μL
Shipping Condition Dry Ice, 2–8°C
Target Kit Named Alkaline Phosphatase (ALP)
Total Assay Time 30 min.
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