Invitrogen™ Alkaline Phosphatase (ALP) Activity Kit

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Alkaline Phosphatase (ALP) Activity Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ALP activity in various sample types. ALP is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase where each catalytic site contains three metal ions.

The assay is based on the ability of alkaline phosphatase to decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol reacts with 4-aminopyrine in alkaline solution and oxidizes with potassium ferricyanide to form a red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.13–50 King Units/100 mL
• Assay Sensitivity: 0.13 King Units/100 mL
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Plasma, Serum, Tissue Homogenates, Whole Cells
• For Use With (Equipment): Microplate Reader
• Incubation Time: 15 min.
• Interassay CV: 8.5
• Intraassay CV: 5.1
• Quantity: 96 tests
• Sample Volume: 5 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Alkaline Phosphatase (ALP)
• Total Assay Time: 30 min.