Invitrogen™ Alkaline Phosphatase (ALP) Activity Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

Supplier:  Invitrogen™ EEA002

Catalog No. EEA002

Description

The Alkaline Phosphatase (ALP) Activity Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ALP activity in various sample types. ALP is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase where each catalytic site contains three metal ions.

The assay is based on the ability of alkaline phosphatase to decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol reacts with 4-aminopyrine in alkaline solution and oxidizes with potassium ferricyanide to form a red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.13–50 King Units/100 mL
• Content And Storage: Store at 2–8°C.
  
  
• Sample Type: Plasma, Serum, Tissue Homogenates, Whole Cells
• Incubation Time: 15 min.
• Intraassay CV: 5.1
• Sample Volume: 5 μL
• Target Kit Named: Alkaline Phosphatase (ALP)

• Assay Sensitivity: 0.13 King Units/100 mL
• Detection Method: Colorimetric
• For Use With (Equipment): Microplate Reader
• Interassay CV: 8.5
• Quantity: 96 tests
• Shipping Condition: Dry Ice, 2–8°C
• Total Assay Time: 30 min.