Invitrogen™ Aspartate Aminotransferase (AST/GOT) Activity Kit

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Aspartate Aminotransferase (AST/GOT) Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of aspartate aminotransferase activity in various sample types. AST/GOT is a key enzyme in nitrogen metabolism, which is widely found in plasma and body tissues, including liver, heart, skeletal muscle, kidney, brain, pancreas, lung, and erythrocytes. Changes in AST/GOT activity were found in acute pancreatitis, ischemic stroke, severe burns, periodontitis, acute renal disease, and motor neuron disease.

The assay is based on the ability of AST/GOT to enable alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form pyroracemic acid during the reaction. Pyroracemic acid reacts with 2,4-dinitro phenyl hydrazine (DNPH) to form 2,4, dinitrophenylhydrazone, showing reddish-brown color in alkaline solution. The OD values can be measured to calculate the enzyme activity.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 1.1–72.3 IU/L
• Assay Sensitivity: 1.1 IU/L
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates
• For Use With (Equipment): Microplate Reader
• Incubation Time: 50 min.
• Interassay CV: 6.8
• Intraassay CV: 5.3
• Quantity: 96 tests
• Sample Volume: 10 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Aspartate Aminotransferase (AST/GOT)
• Total Assay Time: 80 min.