Invitrogen™ Glutathione Peroxidase (GSH-Px) Activity Kit

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Glutathione Peroxidase (GSH-Px) Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of GSH-Px activity in various sample types. GSH-Px is a crucial enzyme that plays a key role in protecting cells against oxidative damage by catalyzing the reduction of hydrogen peroxide and organic hydroperoxides using glutathione as a cofactor, protecting cell membrane structure and keeping membrane function integrity.

The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which presents with a stable yellow color. GSH can be calculated by measuring the absorbance of the anions in solution at 412 nm.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 17.17–518.32 U/L
• Assay Sensitivity: 17.17 U/L
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Whole Cells
• For Use With (Equipment): Microplate Reader
• Incubation Time: 10 min.
• Interassay CV: 6.2
• Intraassay CV: 2.4
• Quantity: 96 tests
• Sample Volume: 40 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Glutathione Peroxidase (GSH-Px)
• Total Assay Time: 40 min.