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Invitrogen™ H2S Colorimetric Assay Kit
Description
The Hydrogen Sulfide (H2S) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of H2S in serum, plasma, and tissue samples. H2S is a crucial signal molecule involved in various biological processes, from cellular signal transduction to nerve conduction and protein synthesis, where it plays a role in regulating development, angiogenesis, and protecting organs from ischemia or reperfusion injury.
In this assay H2S reacts with an acetate solution to create soluble ZnS, where, in the presence of Fe3+, methylene blue is formed (which has a maximum absorption peak at 665 nm). The concentration of iron can be indirectly calculated by measuring the OD value.
Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results
Specifications
Specifications
| Assay Range | 4.86–100 μmol/L |
| Assay Sensitivity | 2.75 μmol/L |
| Content And Storage | Store at 2–8°C. |
| Detection Method | Colorimetric |
| Sample Type | Plasma, Serum, Tissue Homogenates |
| For Use With (Equipment) | Microplate Reader |
| Incubation Time | 20 min. |
| Interassay CV | 9.9 |
| Intraassay CV | 3.1 |
| Quantity | 96 tests |
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