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Invitrogen™ H2S Colorimetric Assay Kit

Catalog No. EEA011
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96 tests
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EEA011 96 tests
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Catalog No. EEA011 Supplier Invitrogen™ Supplier No. EEA011
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These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Hydrogen Sulfide (H2S) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of H2S in serum, plasma, and tissue samples. H2S is a crucial signal molecule involved in various biological processes, from cellular signal transduction to nerve conduction and protein synthesis, where it plays a role in regulating development, angiogenesis, and protecting organs from ischemia or reperfusion injury.

In this assay H2S reacts with an acetate solution to create soluble ZnS, where, in the presence of Fe3+, methylene blue is formed (which has a maximum absorption peak at 665 nm). The concentration of iron can be indirectly calculated by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 4.86–100 μmol/L
Assay Sensitivity 2.75 μmol/L
Content And Storage Store at 2–8°C.

Detection Method Colorimetric
Sample Type Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Microplate Reader
Incubation Time 20 min.
Interassay CV 9.9
Intraassay CV 3.1
Quantity 96 tests
Sample Volume 100 μL
Shipping Condition Dry Ice, 2–8°C
Target Kit Named Hydrogen Sulfide (H2S)
Total Assay Time 80 min.
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