Invitrogen™ Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

Catalog No.	Quantity
EEA017	96 tests

Catalog No. EEA017 Supplier Invitrogen™ Supplier No. EEA017

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of NEFA in animal tissue samples. NEFAs are derived from adiopose tissue, where hormone-sensitive lipase decomposes triglycerides to product fatty acids. These acids circulate freely in the body and, when combined with plasma albumin, can be used as an energy sourced for muscles, organs, and the brain. They are also a substrate of fat synthesis, where the concentration of NEFAs is related to metabolism and endocrine function.

In this assay NEFA react with nantokite to form copper soap, which has a specific absorption peak at 715 nm. The content of NEFA can be calculated indirectly by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.15–1.5 mmol/L
• Assay Sensitivity: 0.15 mmol/L
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Tissue Homogenates
• For Use With (Equipment): Microplate Reader
• Incubation Time: 3 min.
• Interassay CV: 5.1
• Intraassay CV: 3.3
• Quantity: 96 tests
• Sample Volume: 1000 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Non-esterified Free Fatty Acids (NEFA/FFA)
• Total Assay Time: 30 min.