Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Reactive Oxygen Species (ROS) Fluorometric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ROS in fresh tissue and live cell samples. ROS are highly reactive molecules that play a crucial role in various physiological and pathological processes, including oxidative stress, inflammation, and cellular signaling.

The assay works by utilizing the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) to detect the presence of ROS. DCFH-DA is taken up by cells and is converted to dichlorofluorescein (DCFH) by intracellular esterase. DCFH is oxidized by ROS to the strong fluorescent DCF. The fluorescence of DCF (Ex/Em 502/525 nm) is directly proportional to the amount of ROS present in the sample.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Content And Storage: Reagent 1: –20°C
  Reagent 2: 2–8°C
  
  
• Detection Method: Fluorescence
• Sample Type: Cell Culture, Live Tissue
• For Use With (Equipment): Spectrophotometer, Microplate Reader, Flow Cytometer
• Incubation Time: 30-60 min.
• Quantity: 96 tests
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Reactive Oxygen Species (ROS)
• Total Assay Time: 60 min.