Invitrogen™ Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit

Catalog No.	Quantity
EEA021	96 tests

Catalog No. EEA021 Supplier Invitrogen™ Supplier No. EEA021

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of TBARS in serum, plasma, animal tissue, cultured cells, and other sample types. TBARS are products of lipid peroxidation, a process that occurs when polyunsaturated fatty acids in cellular membranes are attacked by oxygen free radicals. TBARS, particularly malondialdehyde (MDA), are commonly used as biomarkers of lipid peroxidation and can reflect the degree of oxidative stress and cellular injury in organisms.

In this assay, TBARS and thiobarbituric acid (TBA) react under high temperature and acidic conditions. This reaction forms a pink compound, which has an optical density measured at 530–540 nm and the concentration of which is linearly related to the concentration of TBARS in the sample.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 2.6–100 μmol/L
• Assay Sensitivity: 0.85 μmol/L
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Plasma, Serum, Tissue Homogenates
• For Use With (Equipment): Microplate Reader
• Incubation Time: 60 min.
• Interassay CV: 7.1
• Intraassay CV: 4.3
• Quantity: 96 tests
• Sample Volume: 100 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Thiobarbituric Acid Reactants (TBARS)
• Total Assay Time: 80 min.