Invitrogen™ Total Antioxidant Status (TAS) Colorimetric Assay Kit

Catalog No.	Quantity
EEA025	96 tests

Catalog No. EEA025 Supplier Invitrogen™ Supplier No. EEA025

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Total Antioxidant Status (TAS) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of TAS in serum, plasma, urine, cellular supernatant, and animal and plant tissue samples. Antioxidants are substances that help protect cells against damage caused by free radicals, which are unstable molecules that can lead to oxidative stress and various diseases..

The assay uses ABTS, which is oxidized to ABTS^•+ (a blue-green solution) and then further reduced back to colorless ABTS in the presence of antioxidants. The total antioxidant status can be calculated by measuring the absorbance of ABTS^•+ at 660 nm.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.29–25.85 mmol/L
• Assay Sensitivity: 0.23 mmol Trolox Equiv./L
• Content And Storage: Store at –20°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Urine
• For Use With (Equipment): Microplate Reader
• Incubation Time: 5 min.
• Interassay CV: 7
• Intraassay CV: 4.6
• Quantity: 96 tests
• Sample Volume: 10 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Total Antioxidant Status (TAS)
• Total Assay Time: 30 min.