MilliporeSigma™ HRV 3C Protease

Description

Highly efficient fusion tag removal at low temperature

• Recombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease
• HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln↓GlyPro
• The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His•Tag™ fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags
• The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, S•Tag™, thioredoxin (Trx•Tag™), glutathione-S-transferase (GST•Tag™), and NusA (Nus•Tag™) sequences
• The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind™ metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences
• Other expression vectors, including the pET-52b(+) vector, the InsectDirect™ pIEx™-9 vector, and the multisystem pTriEx™-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag™ II and His•Tag sequences
• Supplied as: 1.8-3.0U/μl in 50mM Tris-HCl (pH 8.0), 150mM NaCl, 1mM EDTA, 0.5mM THP, and 50% glycerol

Specifications

• Formulation: In 50mM Tris-HCl (pH 8.0), 150mM NaCl, 1mM EDTA, 0.5mM THP, and 50% glycerol
• Content And Storage: -20°C
• Quantity: 500 U
• Product Type: Protease