MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys14)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-100-51MI	25 Assays	Unconjugated

Catalog No. 17-100-51MI Supplier MilliporeSigma™ Supplier No. 1710051

Description

Rabbit Monoclonal Antibody

Specifically detects Acetyl-Histone H3 (Lys14)α Clone: in Human, Rat samples, and it is validated for ChIP, ChIP-seq, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: Acetyl-Histone H3 (Lys14)α
• Applications: ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50μL of unpurified Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Nornal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. ChIP Primers, c-myc promoter. One vial containing 75μL of 5μM of each primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC
• Gene Accession No.: P84243
• Gene Symbols: H3.3A, H3.3B, H3F3, MGC87782, MGC87783, OTTHUMP00000035618, OTTHUMP00000035619, OTTHUMP00000035621
• Host Species: Rabbit
• Immunogen: Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.
• Purification Method: Unpurified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484.1
• Test Specificity: Recognizes Histone H3 acetylated on Lys14.
• Includes: This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Rat
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Isotype: IgG

For Research Use Only