MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys18), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-101-11MI	25 Assays	Unconjugated

Catalog No. 17-101-11MI Supplier MilliporeSigma™ Supplier No. 1710111

Description

Rabbit Polyclonal Antibody

Specifically detects Acetyl-Histone H3 (Lys18) Clone: in Human, S. cerevisiae, Vertebrate, Yeast samples, and it is validated for ChIP, ChIP-seq, Dot Blot, Western Blotting

Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specifications

• Antigen: Acetyl-Histone H3 (Lys18)
• Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Dot Blot, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Acetyl-Histone H3 (Lys18) (rabbit polyclonal). One vial of 50μL of rabbit serum containing 0.05% sodium azide and 30% glycerol. Normal Rabbit Serum. One vial of 100μL of antiserum containing 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM each of primer specific for human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: Q16695
• Gene Symbols: HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3T, H3/g, H3.4, H3/t
• Host Species: Rabbit
• Immunogen: KLH-conjugated, synthetic peptide (GKAPRAcKQLASK-C) corresponding to amino acids 13-23 of yeast Histone H3 acetylated on lysine 18with a C-terminal cysteine added for conjugation purposes
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003493
• Test Specificity: Recognizes Histone H3 acetylated on lysine 18.
• Includes: This ChIPAb+ Acetyl-Histone H3 (Lys18) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, S. cerevisiae, Vertebrates, Yeast
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Serum

For Research Use Only