MilliporeSigma™ Upstate™ Acetyl Histone H3 (Lys23), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-101-12MI	25 Assays	Unconjugated

Catalog No. 17-101-12MI Supplier MilliporeSigma™ Supplier No. 1710112

Description

Rabbit Polyclonal Antibody

Specifically detects Acetyl Histone H3 (Lys23) Clone: in Human, S. cerevisiae, Vertebrate, Yeast samples, and it is validated for ChIP, Immunoprecipitation, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: Acetyl Histone H3 (Lys23)
• Applications: ChIP Assay, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Acetyl Histone H3 (Lys23) (rabbit polyclonal). One vial of 50μL of rabbit serum containing 0.05% sodium azide and 30% glycerol. Normal Rabbit Serum. One vial of 100μL of antiserum containing 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM each of primer specific for human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: Q16695
• Gene Symbols: HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3T, H3/g, H3.4, H3/t
• Host Species: Rabbit
• Immunogen: Ovalbumin-conjugated, synthetic peptide (KQLAS_AcKAARK-C) corresponding to amino acids 18-27 of yeast histone H3 acetylated on lysine 23 with a C-terminal cysteine added for conjugation purposes
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003493
• Test Specificity: Histone H3 acetylated on lysine 23. Does not recognize unacetylated recombinant histone H3
• Includes: This ChIPAb+ Acetyl Histone H3 (Lys23) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, S. cerevisiae, Vertebrates, Yeast
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Serum

For Research Use Only