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MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys27)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No. 17683MI Shop All MilliporeSigma Products
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Quantity:
25 Assays
Conjugate:
Unconjugated
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17-683-MI 25 Assays Unconjugated
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Catalog No. 17-683-MI Supplier MilliporeSigma™ Supplier No. 17683
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Description

Mouse Monoclonal Antibody

Specifically detects Acetyl-Histone H3 (Lys27)α Clone: in Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Lysine acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation and acetylation of H3K27 is found at active enhancers.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Acetyl-Histone H3 (Lys27)α
Applications ChIP Assay, Western Blot
Classification Monoclonal
Conjugate Unconjugated
Formulation Anti-acetyl-Histone H3 (Lys27) (mouse monoclonal IgG1, Clone CMA309). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, RPL10 Promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human RPL10. FOR: ACC CGT CTT CGA CAG GAC T; REV: GGA ACG GAA GAC GAG AAC AG
Gene Accession No. Q66I33
Host Species Mouse
Immunogen The acetyl-histone H3 (Lys27) purified antibody is made against a synthetic peptide (acetylated at Lys27) corresponding to amino acids 19-37 of histone H3.
Quantity 25 Assays
Regulatory Status RUO
Research Discipline Epigenetics and Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NM_003493
Test Specificity Recognizes histone H3, Mr 17kDa, acetylated at Lys27.
Includes Acetyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Ac.
Target Species Vertebrates
Content And Storage −20°C for up to 12 months; Avoid repeated freeze/thaw cycles
Form Purified
Isotype IgG
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For Research Use Only

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