MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys4), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-100-50MI	25 Assays	Unconjugated

Catalog No. 17-100-50MI Supplier MilliporeSigma™ Supplier No. 1710050

Description

Rabbit Polyclonal Antibody

Specifically detects Acetyl-Histone H3 (Lys4) Clone: in Human samples, and it is validated for ChIP, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term Histone H3 alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Specifications

• Antigen: Acetyl-Histone H3 (Lys4)
• Applications: ChIP Assay, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Acetyl-Histone H3 (Lys4) (rabbit polyclonal). One vial containing 100μL of immunoaffinity purified rabbit polyclonal in storage buffer containing 0.05% sodium azide with 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: Q16695
• Gene Symbols: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
• Host Species: Rabbit
• Immunogen: KLH-conjugated, synthetic peptide containing the sequence …RTAcKQ… in which AcK corresponds to acetyl lysine 4 of human histone H3.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484
• Test Specificity: Recognizes Histone H3 acetylated on Lys4.
• Includes: This ChIPAb+ Acetyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only